Vasculogenesis/Angiogenesis PDF Print

Aim. Vasculogenesis/Angiogenesis research project is focused on advanced human in vitro cell models and drug and chemical testing in cell models. Process of angiogenesis is involved in several physiologic and pathologic processes, e.g. in tumor development, wound healing and in rheumatoid arthritis. Information on this process is needed in the treatment of ischemic tissue, in the development of anti-angiogenic cancer drugs as well as in peripheral and coronary vascular diseases. The search for new anti-angiogenic agents for the treatment of cancer is at present a prominent research area.

Figure. Tubule formation in 2D angiogenesis model

Principle. The vasculogenesis/angiogenesis human in vitro cell model mimics the development of normal human vasculature. In the cell model, endothelial cells are able to form tubular structures and connect to form tubular networks. In the test method, the effect of chemicals on the endothelial cell tubule formation ability is studied. Chemicals may be stimulatory (e.g. certain growth factors) or inhibitory (esim. acetyl salicylic acid, thalidomide, growth factor antibodies) Several different concentrations of test chemicals may be run simultaneously and with several dilutions. The assay is responsive to a variety of angiogenesis inhibitors and inducers.

Status. The validated vasculogenesis/angiogenesis assay is currently used as a routine method in our laboratory for drug and chemical testing.

Next step. To develop, characterize and test advanced 2D and 3D in vitro angiogenesis models for tissue engineering applications and for chemical testing.